Abstract
The inversion(16)(p13;q22) gives rise to chimeric transcripts CBFB-MYH11. To date however, no reports have described the full length coding sequence cloned from patient material or the protein product derived from transcripts. We describe here the cloning and sequencing of the coding region of the fusion gene (type A) from patient cells. The sequence is identical to the included portions of the normal constituent transcripts. We report the study of CBFB and CBFB-MYH11 protein using two anti-CBFB antisera. Twenty-two cases of inv(16) leukemia and a number of other cases of AML were examined. The predicted 70 kDa type A or 95 kDa type D CBFB-MYH11 peptide was detected in 20/22 cases of inv(16) AML. CBFB was expressed as a 21 kDa protein in all samples studied, including hematopoietic cell lines of all major lineages.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Chromosome Inversion
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Cloning, Molecular
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Core Binding Factor beta Subunit
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics
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Humans
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Leukemia, Myelomonocytic, Acute / genetics
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Leukemia, Myelomonocytic, Acute / metabolism
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Molecular Sequence Data
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Oncogene Proteins, Fusion / analysis
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Oncogene Proteins, Fusion / biosynthesis
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Oncogene Proteins, Fusion / genetics*
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Open Reading Frames
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Polymerase Chain Reaction
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Transcription Factor AP-2
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Transcription Factors / analysis
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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CBFB protein, human
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CBFbeta-MYH11 fusion protein
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Core Binding Factor beta Subunit
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DNA-Binding Proteins
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Oncogene Proteins, Fusion
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Transcription Factor AP-2
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Transcription Factors