Immunophenotypic and molecular genetic characterization of a case of CD8+ B cell chronic lymphocytic leukemia

Leukemia. 1996 Sep;10(9):1544-50.

Abstract

We report the case of a patient with chronic B lymphocytic leukemia, with stable clinical and hematological conditions in the absence of any treatment. The flow cytometry analysis of the patient's cells revealed a CD19+, CD5+, IgM+, IgD+, lambda chain-phenotype, along with an unusual expression of CD8 alpha/alpha homodimer. B cells did not stain with the anti CD8 beta monoclonal antibody T8/2T8 5H7. Molecular biology analyses confirmed the monoclonal rearrangements of immunoglobulin heavy and lambda light chain genes in the presence of a germline configuration of T cell receptor genes. Moreover, an abnormal configuration of the CD8A gene was found in the CD8+B lymphocytic clone. We suggest that the aberrant expression of the CD8 gene could be related to its abnormal configuration.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • CD8 Antigens / analysis*
  • CD8 Antigens / biosynthesis
  • CD8 Antigens / genetics
  • DNA, Neoplasm / analysis
  • DNA, Neoplasm / genetics
  • Gene Expression
  • Gene Rearrangement, T-Lymphocyte / genetics
  • Humans
  • Immunophenotyping
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • Leukemia, Lymphocytic, Chronic, B-Cell / immunology
  • Leukemia, Lymphocytic, Chronic, B-Cell / metabolism*
  • Male
  • Middle Aged
  • Molecular Sequence Data

Substances

  • CD8 Antigens
  • DNA, Neoplasm