The study was carried out for reviewing the hypothesis on the relationship between integrated fish farming (IFF) and human influenza A pandemic using study methods of longitudinal epidemiology, ecology and serology. Four rural villages with IFF, non-IFF and non-fish farming, selected from two provinces (Henan and Shandong of PRC) were investigated for the status about human influenza A epidemic and infections with human, duck, swine influenaz A viruses in those communities. The results put forward views on that there is no relation between IFF and the increase of incidence rate, infection rate and antibodies level to human influenza A virus. Duck may play an important role in the increase of infection rate to human influenza A viruses among human communities. Pigs seem not to play obvious role in the increase of infection rate to human influenza A viruses among human communities. Human, duck, swine influenza A viruses may spread among human duck and pigs communities interchangably.