Abstract
The effects of PACAP on c-fos mRNA using small cell lung cancer (SCLC) cell was investigated. PACAP-27 (100 nM) increased c-fos mRNA 5-fold using NCI-N417 cells. The increase was concentration dependent with 0.1 nM PACAP-27 half maximally increasing c-fos mRNA. Also the increase in c-fos mRNA caused by PACAP was time dependent; being maximal after 1 hour and returning to basal values after 4 hours. PACAP-38 but not PACAP(28-38) increased c-fos mRNA. One uM PACAP(6-38), a PACAP receptor antagonist, inhibited the increase in c-fos mRNA caused by 1 nM PACAP. These data indicate that PACAP stimulates nuclear oncogene expression in SCLC cells.
MeSH terms
-
Carcinoma, Small Cell
-
Dose-Response Relationship, Drug
-
Genes, fos / drug effects*
-
Humans
-
Kinetics
-
Lung Neoplasms
-
Neuropeptides / pharmacology*
-
Neurotransmitter Agents / pharmacology
-
Peptide Fragments / pharmacology
-
Pituitary Adenylate Cyclase-Activating Polypeptide
-
Proto-Oncogene Proteins c-fos / biosynthesis*
-
Proto-Oncogene Proteins c-fos / genetics
-
RNA, Messenger / biosynthesis
-
Transcription, Genetic / drug effects*
-
Tumor Cells, Cultured
-
Vasoactive Intestinal Peptide / pharmacology
Substances
-
ADCYAP1 protein, human
-
Neuropeptides
-
Neurotransmitter Agents
-
Peptide Fragments
-
Pituitary Adenylate Cyclase-Activating Polypeptide
-
Proto-Oncogene Proteins c-fos
-
RNA, Messenger
-
Vasoactive Intestinal Peptide