Abstract
We obtained a human myeloma cell line (XG4-CNTF) whose growth was completely dependent on addition of ciliary neurotrophic factor (CNTF). Half-maximal proliferation was induced by adding 20 pg/mL CNTF. Response to CNTF correlated with expression of membrane CNTF receptor alpha-chain (CNTFR alpha), as shown by PCR analysis and immunostaining with anti-CNTFR alpha antibodies. CNTF-induced proliferation was completely inhibited by antibodies to gp130 interleukin-6 (IL-6) transducer, unlike antibodies to IL-6 or IL-6 receptor (IL-6R). Growth of XG4-CNTF cells using the gp130 IL-6 transducer was also supported by other cytokines: IL-6, leukemia inhibitory factor (LIF), and oncostatin M (OM). This cell line should be very useful for studying the interactions of IL-6-related cytokines with their receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / pharmacology
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Antigens, CD / immunology
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Antigens, CD / physiology
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Cell Division / drug effects*
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Cell Line
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Cell Membrane / drug effects
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Cell Membrane / metabolism
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Ciliary Neurotrophic Factor
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Dose-Response Relationship, Drug
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Gene Expression* / drug effects
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Growth Inhibitors / pharmacology
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Humans
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Interleukin-6 / immunology
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Interleukin-6 / pharmacology
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Interleukin-6 / physiology
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Lymphokines / pharmacology
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Multiple Myeloma
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Nerve Growth Factors / pharmacology
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Nerve Tissue Proteins / pharmacology*
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Polymerase Chain Reaction
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Receptor, Ciliary Neurotrophic Factor
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Receptors, Cytokine / biosynthesis*
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Receptors, Interleukin / immunology
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Receptors, Interleukin / physiology
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Receptors, Interleukin-6
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Receptors, Nerve Growth Factor / biosynthesis*
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Receptors, OSM-LIF
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Tumor Cells, Cultured
Substances
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Antibodies
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Antigens, CD
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Ciliary Neurotrophic Factor
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Growth Inhibitors
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Interleukin-6
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LIF protein, human
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LIFR protein, human
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Lymphokines
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Nerve Growth Factors
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Nerve Tissue Proteins
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Receptor, Ciliary Neurotrophic Factor
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Receptors, Cytokine
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Receptors, Interleukin
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Receptors, Interleukin-6
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Receptors, Nerve Growth Factor
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Receptors, OSM-LIF