Abstract
The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / chemistry
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Antibody Specificity*
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Carbohydrate Sequence
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Gangliosides / immunology*
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Glycolipids / immunology*
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Glycolipids / metabolism
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Humans
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Immunohistochemistry
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Neuraminic Acids / analysis*
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Neuraminic Acids / immunology
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Organ Specificity / immunology
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Sulfoglycosphingolipids / immunology
Substances
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Antibodies, Monoclonal
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Gangliosides
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Glycolipids
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Neuraminic Acids
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Sulfoglycosphingolipids
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N-glycolylneuraminic acid