IgE-anti-IgE-induced prostaglandin D2 release from cultured human mast cells

Biochem Biophys Res Commun. 1996 Aug 23;225(3):1015-20. doi: 10.1006/bbrc.1996.1287.

Abstract

To elucidate the characterization of cultured human mast cells, the generation of prostaglandin D2 (PGD2) upon stimulation with IgE-anti IgE challenge was determined by using gas chromatography/mass spectrometry/selected ion monitoring (GC/MS/SIM) assay. Mononuclear cells obtained from human umbilical cord blood were cultured in the presence of Steel Factor, IL-6, and PGE2. After 8 weeks of culture, approximately 90 to 95% of cultured cells became tryptase-positive and included basophilic granules. At 12 weeks of culture, cells were harvested and incubated with 1 microgram/ml of human IgE for 1 hr and then challenged with 10 micrograms/ml of anti-IgE. The level of PGD2 release into the supernatant was measured by GC/MS/SIM with the stable isotope dilution method. Cyclooxygenase inhibitors affected the PGD2 release from the cell by IgE-anti IgE challenge incubation. Indomethacin inhibited 98% of PGD2 release. Acetylsalicylic acid (aspirin) and NS-398 (potent COX-2 inhibitor) also inhibited PGD2 release by 85 and 45% respectively.

MeSH terms

  • Antibodies, Anti-Idiotypic / pharmacology*
  • Cell Differentiation
  • Cells, Cultured
  • Chymases
  • Fetal Blood / cytology
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Immunoglobulin E / pharmacology*
  • Immunohistochemistry
  • Mast Cells / immunology*
  • Mast Cells / metabolism*
  • Mast Cells / ultrastructure
  • Prostaglandin D2 / metabolism*
  • Serine Endopeptidases / metabolism
  • Tryptases

Substances

  • Antibodies, Anti-Idiotypic
  • Immunoglobulin E
  • Serine Endopeptidases
  • chymase 2
  • Chymases
  • Tryptases
  • Prostaglandin D2