Abstract
TEM-28 (pI 6.1), expressed by an Escherichia coli clinical isolate, is a novel beta-lactamase which hydrolyzed ceftazidime, cefotaxime, and aztreonam with rates of 25, 1.1, and 5.6, respectively, relative to that for benzylpenicillin (100). The nucleotide sequence of blaTEM-28 differed from that of blaTEM-1 by two base changes, resulting in amino acid substitutions of Arg-164 to His and Glu-240 to Lys.
MeSH terms
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Drug Resistance, Microbial
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Escherichia coli / isolation & purification
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Escherichia coli Infections / enzymology*
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Escherichia coli Infections / microbiology*
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Isoelectric Focusing
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Plasmids
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Transformation, Genetic
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beta-Lactamases / genetics
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beta-Lactamases / isolation & purification*
Substances
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beta-Lactamases
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beta-lactamase TEM-1