Abstract
Tissue-specific transcription is regulated in part by cell type-restricted proteins that bind to defined sequences in target genes. The DNA-binding domain of these proteins is often evolutionarily conserved. On this basis, liver-enriched transcription factors were classified into five families. We describe here the mammalian prototype of a sixth family, which we therefore call hepatocyte nuclear factor 6 (HNF-6). It activates the promoter of a gene involved in the control of glucose metabolism. HNF-6 contains two different DNA-binding domains. One of these corresponds to a novel type of homeodomain. The other is homologous to the Drosophila cut domain. A similar bipartite sequence is coded by the genome of Caenorhabditis elegans.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Affinity Labels
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Amino Acid Sequence
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Animals
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Autoradiography
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Base Sequence
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Binding Sites
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Caenorhabditis elegans / genetics
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Cloning, Molecular
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DNA / metabolism
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Drosophila / genetics
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Electrophoresis, Polyacrylamide Gel
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Glucose / metabolism
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Hepatocyte Nuclear Factor 6
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / genetics
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Homeodomain Proteins / isolation & purification*
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Molecular Sequence Data
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Promoter Regions, Genetic
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Rats
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Sequence Homology, Nucleic Acid
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Trans-Activators / isolation & purification*
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / isolation & purification*
Substances
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Affinity Labels
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Hepatocyte Nuclear Factor 6
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Homeodomain Proteins
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Onecut1 protein, rat
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Trans-Activators
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Transcription Factors
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DNA
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Glucose
Associated data
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GENBANK/U77975
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GENBANK/X96553