Abstract
Nordihydroguaiaretic acid (NDGA) blocks intra-Golgi protein transport in a cell-free system and prolactin secretion from GH3 cells [Tagaya, M., Henomatsu, N., Yoshimori, T., Yamamoto, A., Tashiro, Y., and Fukui, T. (1993) FEBS Lett. 324, 201-204]. To determine which intracellular secretory pathway(s) is inhibited by NDGA, we investigated its effect on the transport of the vesicular stomatitis virus-encoded glycoprotein in BHK-21 cells. NDGA blocked protein transport from the endoplasmic reticulum to the Golgi apparatus, and from the trans-Golgi network to the plasma membrane. In addition, it retarded the brefeldin A-induced retrograde transport of mannosidase II to the endoplasmic reticulum. Although NDGA had an inhibitory effect on protein synthesis, it induced the expression of BiP, a chaperone located in the endoplasmic reticulum. The induction of BiP may be a consequence of the inhibition of protein transport by NDGA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biological Transport / drug effects
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Brefeldin A
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Carrier Proteins / biosynthesis
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Cell Line
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Cell Membrane / drug effects
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Cell Membrane / metabolism*
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Cricetinae
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Cyclopentanes / pharmacology
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Endoplasmic Reticulum / drug effects
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Endoplasmic Reticulum / metabolism*
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Endoplasmic Reticulum / ultrastructure
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Endoplasmic Reticulum Chaperone BiP
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Glycoproteins / metabolism
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Golgi Apparatus / drug effects
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Heat-Shock Proteins*
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Mannosidases / metabolism*
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Masoprocol / pharmacology*
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Membrane Glycoproteins*
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Microtubules / drug effects
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Microtubules / metabolism
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Microtubules / ultrastructure
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Molecular Chaperones / biosynthesis
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Vesicular stomatitis Indiana virus / physiology
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Viral Envelope Proteins / metabolism*
Substances
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Carrier Proteins
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Cyclopentanes
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Endoplasmic Reticulum Chaperone BiP
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G protein, vesicular stomatitis virus
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Glycoproteins
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Heat-Shock Proteins
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Membrane Glycoproteins
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Molecular Chaperones
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Viral Envelope Proteins
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Brefeldin A
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Masoprocol
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Mannosidases
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mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase