Molecular cloning of GAF2, a Schizosaccharomyces pombe GATA factor, which has two zinc-finger sequences

Biochem Mol Biol Int. 1996 May;39(1):127-35. doi: 10.1080/15216549600201131.

Abstract

By low stringency screening of a lambda-Shizosaccharomyces pombe genomic library, we have cloned a GATA factor homologous gene, gaf2+, within a 3.1-kb EcoRI fragment. The gaf2 ORF predicts a protein of M(r) 61 kDa consisting of intronless 564 amino acids corresponding to 1,692 bp. Gaf2 has two zinc-fingers as Urbs1 of Ustilago maydis, whereas most of fungal GATA factors have only one zinc-finger. The separation between two zinc-fingers of Gaf2 is rather long. In addition to gaf2, the sequence analysis revealed a Val-tRNA gene in the 3'-flanking region of gaf2. Northern blot analysis indicated that the gaf2 gene is transcribed constitutively irrespective of the nitrogen source in a medium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • GATA Transcription Factors
  • Genes, Fungal
  • Molecular Sequence Data
  • Open Reading Frames
  • RNA, Transfer, Val / analysis
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins*
  • Transcription Factors / genetics*
  • Zinc Fingers

Substances

  • DNA-Binding Proteins
  • Fep1 protein, S pombe
  • GATA Transcription Factors
  • RNA, Transfer, Val
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors

Associated data

  • GENBANK/L29051