A transcription map of the major histocompatibility complex (MHC) class I region

Genomics. 1996 Aug 15;36(1):70-85. doi: 10.1006/geno.1996.0427.

Abstract

We have applied cDNA hybridization selection to nine YACs spanning 3 Mb of genomic DNA from a region centromeric to HLA-A to the histone cluster that lies telomeric to the human major histocompatibility complex (MHC). In addition to Class I genes and pseudogenes, we describe over 63 genes and 23 additional expressed sequence tags distributed throughout the region. Many of the full-length genes belong to gene families. Prominent among these are a group of genes encoding proteins showing homology to the carboxyl-terminal sequences of butyrophilin and an additional group of zinc finger genes. We also detected several previously undefined genes that are specifically expressed in cells of the immune system, indicating a more complex role of the MHC in the immune response than has been appreciated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping / methods*
  • Chromosomes, Artificial, Yeast / genetics
  • DNA, Complementary / genetics*
  • Evolution, Molecular
  • Gene Expression
  • Genes, MHC Class I / genetics*
  • Humans
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Pseudogenes / genetics
  • RNA, Messenger / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*
  • Zinc Fingers

Substances

  • DNA, Complementary
  • RNA, Messenger

Associated data

  • GENBANK/A42604
  • GENBANK/D29763
  • GENBANK/D31762
  • GENBANK/G06384
  • GENBANK/M88579
  • GENBANK/X57352
  • GENBANK/Z11773