Abstract
A total of 0.3 to 0.4 kb of the promoter region of the 16S rRNA genes from Mycobacterium tuberculosis, M. gordonae, M. xenopi, and M. leprae was PCR amplified, cloned, and sequenced. The observed number of substitutions, insertions, and deletions exceeded those found in previously used target sequences, including the entire 16S coding region. A simple and generally applicable restriction fragment length polymorphism method that can be used to distinguish between mycobacterial species is described.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA, Bacterial / genetics
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DNA, Ribosomal / genetics
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Genes, Bacterial*
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Humans
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Molecular Sequence Data
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Mycobacterium / classification
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Mycobacterium / genetics*
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Mycobacterium / isolation & purification
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Mycobacterium leprae / genetics
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Mycobacterium tuberculosis / genetics
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Nontuberculous Mycobacteria / classification
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Nontuberculous Mycobacteria / genetics
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Promoter Regions, Genetic*
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RNA, Bacterial / genetics*
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RNA, Ribosomal, 16S / genetics*
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Sequence Homology, Nucleic Acid
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Species Specificity
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Bacterial
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RNA, Ribosomal, 16S
Associated data
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GENBANK/U17935
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GENBANK/U17957
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GENBANK/U17959