Twenty-four nursing college students aged 16 and 17 years were selected as research subjects and divided into two groups. Group A comprised 12 individuals who were trained for short distance running (5km/day) over a four-week period, while group B was trained for middle distance running (7km/day) during the same period. Blood AST (aspartate aminotransferase), ALT (alanine aminotransferase) and lactate were performed at rest (before training) and after exercise every week. After both short and middle distance exercise training, the lactate values after 1-3 week(s) training period were persistently higher than those before training and the differences between then are significant. However, the lactate value after 4 weeks training period is lower than that after the third week training. There are significant differences between the AST values after 1-4 week(s) training period and those before exercise in short distance exercise training. There are no significant differences between the AST values after training and those before exercise in middle distance exercise training. The ALT values after 1-4 week(s) training period were lower than those before exercise in short and middle distance exercise training. In conclusion, after 4 weeks training, the lactate and AST values can't reduce to those before training in middle distance exercise training, and the lactate value in short distance exercise training is the same as former. Further investigation is needed.