Abstract
A search for new genes was performed in a 220-kb region around the tumor necrosis factor gene cluster in the human central major histocompatibility complex region using a cDNA hybridization and selection method. In addition to the seven known genes in this region, we identified a new gene that is preferentially expressed in spleen. We also identified two pseudogenes that have high degrees of homology to cytokeratin and cyclophillin, respectively. Expressed sequences for a human homologue of the mouse B144 gene were also found in the current analysis. RT-PCR analysis showed that B144 is expressed in spleen, in thymus, and prominently in the macrophage cell line, U937. We also independently identified the BAT1 gene to be the well-conserved homologue of a previously described rat liver nuclear protein.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blood Proteins / genetics
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CHO Cells
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Cricetinae
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DNA, Complementary / genetics
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Gene Library
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Genes, MHC Class I
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Genome, Human
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Humans
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Intracellular Signaling Peptides and Proteins
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Keratins / genetics
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Lymphotoxin-alpha / genetics
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Lymphotoxin-beta
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Major Histocompatibility Complex / genetics*
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Membrane Proteins / genetics
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Mice
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Molecular Sequence Data
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Multigene Family / genetics*
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Proto-Oncogene Proteins / genetics
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Rats
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Receptors, Immunologic / genetics
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Transcription Factor RelB
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Transcription Factors*
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Tumor Necrosis Factor-alpha / genetics*
Substances
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Blood Proteins
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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LST1 protein, human
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LTB protein, human
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Lst1 protein, mouse
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Ltb protein, mouse
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Ltb protein, rat
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Lymphotoxin-alpha
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Lymphotoxin-beta
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Membrane Proteins
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Proto-Oncogene Proteins
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RELB protein, human
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Receptors, Immunologic
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Relb protein, mouse
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Relb protein, rat
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Transcription Factors
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Tumor Necrosis Factor-alpha
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cyclosporin receptor
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Transcription Factor RelB
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Keratins
Associated data
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GENBANK/AF031136
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GENBANK/AF031137
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GENBANK/AF031138