Autogenous control of the suhB gene expression of Escherichia coli

Biochimie. 1996;78(3):209-12. doi: 10.1016/0300-9084(96)89508-3.

Abstract

The suhB gene encodes a 29-kDa protein that possesses inositol monophosphate activity and its mutations suppress several temperature-sensitive mutations in Escherichia coli. We found that the suhB gene is autoregulated; suhB- mutant strains oversynthesized the suhB mRNA and suhB-lacZ gene/operon fusion products. Since the half-life of the suhB transcript in the suhB mutant was much greater than the half-life in the wildtype strain, the suhB protein is implicated in the control of gene expression by modulating mRNA turnover.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Gene Expression Regulation, Bacterial / physiology*
  • Genes, Bacterial / genetics
  • Homeostasis
  • Mutation
  • Phosphoric Monoester Hydrolases / genetics*
  • RNA, Bacterial / metabolism
  • RNA, Messenger / metabolism
  • Recombinant Fusion Proteins / biosynthesis

Substances

  • RNA, Bacterial
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Phosphoric Monoester Hydrolases
  • myo-inositol-1 (or 4)-monophosphatase