Abstract
To promote application of a single chain variable region fragment (sFv) in immunoglobulins, a sFv gene was connected to an IgGi Fc gene, designated as a sFvc gene, and used for transfection of Sp2/0. As a result, the sFvc protein was found to be secreted in a dimeric form. It is thus felt that the sFvc protein, which mimicks the shape of a naturally occurring antibody, can be simple and useful to reproduce divalency and Fc-associated effecter functions as seen in a natural antibody.
MeSH terms
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Animals
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Cloning, Molecular
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Dimerization
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Genes, Immunoglobulin / genetics
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Genetic Vectors / genetics
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Humans
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Hybridomas / metabolism*
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Immunoglobulin Fc Fragments / genetics
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Immunoglobulin Fragments / chemistry
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Immunoglobulin Fragments / genetics*
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Variable Region / genetics*
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Immunoglobulin kappa-Chains / genetics
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Mice
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Recombinant Proteins* / genetics
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Recombinant Proteins* / metabolism
Substances
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Immunoglobulin Fc Fragments
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Immunoglobulin Fragments
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Immunoglobulin Heavy Chains
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Immunoglobulin Variable Region
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Immunoglobulin kappa-Chains
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Recombinant Proteins
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immunoglobulin Fv