Despite numerous advances in graft fixation with anterior cruciate ligament (ACL) reconstruction, few studies have compared the fixation strength of interference screws placed "outside-in" and from "inside-out" techniques. To compare techniques, a bovine model was designed to fail at the femoral tunnel bone-screw interface. Twenty-four fresh bovine knees were stripped of all soft tissues except the ACL. The native ACL was loaded the failure at a strain rate of 50 cm/min with the knee flexed 45 degrees. One standardized femoral tunnel was created on all specimens. A 3/32-inch guide pin was drilled into the center of the ACL femoral origin and overreamed with an 11-mm reamer from inside-out until the lateral cortex was reamed. Consistently sized patellar bone blocks were created (8 x 5 x 25 mm) with an 8-mm tendon width. The bone blocks were randomized to an "inside-out" (group 1) and "outside-in" (group 2) technique. Bone blocks were secured with a 7 x 25 interference screw. Specimens were mounted with the femoral tunnel and bone block aligned parallel to the tensile force and strained to failure at 50 cm/min. Failure of the native bovine ACL occurred at a mean of 2,304 N (SD +/- 472 N; n = 24). The mode of failure for group 1 was 9 of 13 at the bone-screw interface and 4 of 13 interligamentous failures. The mode of failure for group 2 was 7 of 11 at the bone-screw interface, 3 of 11 interligamentous, and 1 bone block failure. The mean load to failure for group 1 was 1,151 N (SD +/- 320 N, n = 13) including the four ligamentous failures and 1,143 N (SD +/- 306 N, n = 9) excluding the ligamentous failures. The mean load to failure for group 2 was 1,017 N (SD +/- 262 N, n = 11), including all specimens and 843 N (SD +/- 262 N, n = 7) excluding the interligamentous and bone block failure specimens. The "inside-out" technique averaged 100 N greater fixation strength than the "outside-in" technique. Statistical analysis using two-sample Student's t-test showed no statistically significant differences between group 1 and group 2. Both techniques demonstrate comparable maximum load to failure in a bovine model tested at 50 cm/min.