Osmotic scan ektacytometry in clinical diagnosis

J Pediatr Hematol Oncol. 1996 May;18(2):122-9. doi: 10.1097/00043426-199605000-00005.

Abstract

Purpose: The use of red blood cell deformability measurements in the diagnosis of hemolytic anemia is reviewed.

Patients and methods: Results from 500 individuals are discussed. Erythrocytes were characterized by the automated measurement of cell deformability as the tonicity of the medium is varied (osmotic scan ektacytometry).

Results: The measurement yields a reliable identification of hemolytic anemias caused by deficiencies or abnormalities in erythrocyte structural proteins. In addition, it can, with good reliability, signal the presence of a glycolytic enzyme deficiency. The scan is complete in 15 min, and can therefore give a rapid indication of the type of hemolytic anemia. The osmotic scans of other hemolytic anemias are also discussed.

Conclusion: The analysis of red blood cell deformability can offer a valuable addition to diagnostic methods in hemolytic anemia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Anemia, Hemolytic / blood*
  • Anemia, Hemolytic / diagnosis*
  • Child
  • Erythrocyte Deformability*
  • Hemorheology / methods
  • Humans
  • Osmotic Pressure