Different arrangement of human papillomavirus E2 binding sites distinguishes cutaneous types from those associated with mucosal lesions

Arch Med Res. 1996 Autumn;27(3):389-94.

Abstract

High-risk-type human papillomavirus DNA sequences are found in a high percentage of carcinomas from the uterine-cervix, with the viral E1-E2 gene region usually disrupted and the E6 and E7 oncoproteins consistently expressed. The E2 protein is known to repress early transcription from genital HPV promoters having a proximal E2 binding site (E2BS) close to the TATA box. On the contrary, the E2 protein activates cutaneous early promoters having a longer distance between these sites. Using an in vivo approach we analyzed the regulation, by the BPV-1E2 protein, of a natural HPV-18 promoter where proximal E2BS were placed at variable positions relative to the TATA box, and of heterologous promoters where E2BS was placed upstream of any other known DNA-binding elements. Our results confirm that the E2 protein represses or activates HPV early gene transcription depending on the distance between the TATA box and the proximal E2BS.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Bovine papillomavirus 1 / genetics
  • COS Cells
  • Carcinoma / virology
  • Cattle
  • DNA, Recombinant / genetics
  • DNA, Viral / genetics*
  • DNA-Binding Proteins*
  • Female
  • Gene Expression Regulation, Viral*
  • Humans
  • Mice
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / metabolism*
  • Papillomaviridae / classification
  • Papillomaviridae / genetics*
  • Papillomaviridae / pathogenicity
  • Papillomavirus Infections / virology*
  • Promoter Regions, Genetic*
  • Regulatory Sequences, Nucleic Acid
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Skin Diseases, Viral / virology*
  • TATA Box
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Transfection
  • Tumor Virus Infections / virology*
  • Uterine Cervical Neoplasms / virology
  • Uterine Cervicitis / virology*

Substances

  • DNA, Recombinant
  • DNA, Viral
  • DNA-Binding Proteins
  • E2 protein, Human papillomavirus type 16
  • Oncogene Proteins, Viral
  • Repressor Proteins
  • Transcription Factors
  • oncogene protein E2, Human papillomavirus type 18