Abstract
An mdr1 hammerhead was introduced into two adriamycin-selected multi-drug resistant human lung cell lines both of which over-express p-glycoprotein. Expression of the ribozyme resulted in a decrease in mdr1 mRNA expression and an increase in drug sensitivity in both cell lines. This would suggest that the use of specific ribozymes may represent an effective and specific approach in order to restore cellular sensitivity towards anti-cancer drugs.
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics*
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Antibiotics, Antineoplastic / metabolism
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Antibiotics, Antineoplastic / pharmacology
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Antineoplastic Agents, Phytogenic / metabolism
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Antineoplastic Agents, Phytogenic / pharmacology
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Base Sequence
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology*
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Codon / genetics
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Doxorubicin / pharmacology
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Drug Resistance, Multiple*
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Drug Resistance, Neoplasm*
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Etoposide / pharmacology
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Exons / genetics
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology*
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Neoplasm Proteins / genetics*
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Phenotype
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Plasmids
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Polymerase Chain Reaction
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RNA, Antisense / metabolism
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RNA, Antisense / pharmacology*
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RNA, Antisense / toxicity
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RNA, Catalytic / metabolism
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RNA, Catalytic / pharmacology*
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RNA, Catalytic / toxicity
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RNA, Messenger / drug effects*
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RNA, Messenger / metabolism
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RNA, Neoplasm / drug effects*
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RNA, Neoplasm / metabolism
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Substrate Specificity
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Transfection
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Tumor Cells, Cultured / drug effects
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Vincristine / pharmacology
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antibiotics, Antineoplastic
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Antineoplastic Agents, Phytogenic
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Codon
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Neoplasm Proteins
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RNA, Antisense
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RNA, Catalytic
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RNA, Messenger
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RNA, Neoplasm
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Vincristine
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Etoposide
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Doxorubicin