Structure and organization of the mouse elk1 gene

Gene. 1996 Sep 26;174(1):185-8. doi: 10.1016/0378-1119(96)00357-5.

Abstract

In the ets gene family of transcription factors, elk1 belongs to the subfamily of Ternary Complex Factors (TCFs) which bind to the Serum Response Element (SRE) in conjunction with a dimer of Serum Response Factors (SRFs). In this communication we report the isolation of cDNAs from the mouse elk1 gene, containing the full coding sequence homologous (87% identical) to the human gene, and the structure and organization of 22 kb of the mouse elk1 locus. The coding sequence is spread through 5 exons (numbered 1 to 5): exons 1 to 4 range from 102 bp to 447 bp and exon 5 is at least 620 bp. Exon 0 was not found in the 8.5 kb sequence upstream of exon 1. The intron between exons 1 and 2 is 4 kb long and the 3 other introns are less than 500 bp long. This information will be useful to engineer targeted mutations of this gene in mice and to determine the genomic structure of the other TCF genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • DNA-Binding Proteins*
  • Genome
  • Humans
  • Mice
  • Molecular Sequence Data
  • Proto-Oncogene Proteins / genetics*
  • Sequence Analysis, DNA
  • Transcription Factors / genetics*
  • ets-Domain Protein Elk-1

Substances

  • DNA-Binding Proteins
  • ELK1 protein, human
  • Elk1 protein, mouse
  • Proto-Oncogene Proteins
  • Transcription Factors
  • ets-Domain Protein Elk-1

Associated data

  • GENBANK/X87257