Abstract
Listeria monocytogenes is a bacterial pathogen that invades cultured nonphagocytic cells. Inhibitors and a dominant negative mutation were used to demonstrate that efficient entry requires the phosphoinositide (PI) 3-kinase p85alpha-p110. Infection with L. monocytogenes caused rapid increases in cellular amounts of PI(3, 4)P2 and PI(3,4,5)P3, indicating that invading bacteria stimulated PI 3-kinase activity. This stimulation required the bacterial protein InlB, host cell tyrosine phosphorylation, and association of p85alpha with one or more tyrosine-phosphorylated proteins. This role for PI 3-kinase in bacterial entry may have parallels in some endocytic events.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Androstadienes / pharmacology
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Animals
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Bacterial Proteins / physiology
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Cell Line
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Chromones / pharmacology
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Cytochalasin D / pharmacology
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Enzyme Activation
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Enzyme Inhibitors / pharmacology
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Genistein
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Humans
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Isoflavones / pharmacology
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Listeria monocytogenes / enzymology*
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Listeria monocytogenes / pathogenicity*
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Membrane Proteins / physiology
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Morpholines / pharmacology
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Phosphatidylinositol 3-Kinases
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Phosphatidylinositol Phosphates / metabolism
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Phosphoproteins / metabolism
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Phosphorylation
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Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Phosphotyrosine / metabolism
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Tumor Cells, Cultured
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Wortmannin
Substances
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Androstadienes
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Bacterial Proteins
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Chromones
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Enzyme Inhibitors
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Isoflavones
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Membrane Proteins
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Morpholines
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Phosphatidylinositol Phosphates
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Phosphoproteins
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inlB protein, Listeria monocytogenes
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phosphatidylinositol 3,4,5-triphosphate
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phosphatidylinositol 3,4-diphosphate
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internalin protein, Bacteria
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Phosphotyrosine
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Cytochalasin D
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2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
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Genistein
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Phosphatidylinositol 3-Kinases
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Phosphotransferases (Alcohol Group Acceptor)
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Wortmannin