Previous studies have reported the presence of the calcium binding protein calretinin in neurons in the striatal part of the basal ganglia in rats and primates. In the present study, immunofluorescence double-labeling techniques and immunofluorescence combined with retrograde labeling were used in rats to determine whether calretinin is found in any of the known types of striatal neurons. The results showed that a small fraction of the calretinin-containing neurons (< 10%) contain parvalbumin, but none of the calretinin-containing striatal neurons contained markers for the other two major types of striatal interneurons (i.e., choline acetyltransferase-containing cholinergic neurons and somatostatin-containing neurons). Additionally, calretinin was not found in projection neurons, using either calbindin or DARPP32 as immunofluorescent markers of striatal projections neurons in general, or using retrograde labeling to specifically identify either striatonigral or striatopallidal neurons. Thus, calretinin appears to be largely found in a unique population of striatal interneurons in rats. This population appears to be about one third the abundance of any of the previously identified populations of striatal interneurons.