Urinary excretion of 2,3-dinor-6-keto-PGF1 alpha and 11-dehydro-TXB2 by the gravid spontaneously hypertensive rat

Prostaglandins. 1996 Jul;52(1):1-11. doi: 10.1016/0090-6980(96)00049-4.

Abstract

Little is known about the pathophysiological processes leading to superimposed preeclampsia. We present an animal model where the uteroplacental blood flow in spontaneously hypertensive rats (SHR) was reduced by a silver clip. Thus, a superimposed preeclampsia-like syndrome could be studied under defined conditions. Urinary excretion of 2,3-dinor-6-keto PGF1 alpha and 11-dehydro-TxB2 were measured by enzyme immunoassays at day 16 and 20 of pregnancy. In gravid, sham-operated animals excretion of 2,3-dinor-6-keto-PGF1 alpha was largely elevated compared to non gravid control animals (day 16: 1259 vs. 258 ng/kg 24h; day 20: 471 vs. 269 ng/kg.24h). However, in the gravid rats with reduced uteroplacental blood flow urinary excretion of 2,3-dinor-6-keto-PGF1 alpha decreased to non gravid levels (day 16: 335 ng/kg.24h; day 20: 238 ng/kg.24h). By antihypertensive therapy with dihydralazin this effect was largely abolished. Only minor alterations were found in the excretion of 11-dehydro-TxB2. Our findings suggest, that a reduction of uteroplacental blood flow in the spontaneously hypertensive rat decreases the systemic prostacyclin synthesis.

MeSH terms

  • 6-Ketoprostaglandin F1 alpha / analogs & derivatives*
  • 6-Ketoprostaglandin F1 alpha / urine
  • Animals
  • Antihypertensive Agents / pharmacology
  • Aorta / surgery
  • Blood Pressure / drug effects
  • Dihydralazine / pharmacology
  • Disease Models, Animal
  • Female
  • Placenta / blood supply
  • Placenta / metabolism*
  • Pre-Eclampsia / metabolism*
  • Pre-Eclampsia / urine
  • Pregnancy
  • Rats
  • Rats, Inbred SHR
  • Thromboxane B2 / analogs & derivatives*
  • Thromboxane B2 / urine

Substances

  • Antihypertensive Agents
  • Thromboxane B2
  • 6-Ketoprostaglandin F1 alpha
  • 2,3-dinor-6-ketoprostaglandin F1alpha
  • 11-dehydro-thromboxane B2
  • Dihydralazine