Abstract
To identify the functional P2 purinoceptor subtype in glomerular mesangial cells (GMC), polymerase chain reaction coupled with reverse transcription (RT-PCR) were performed, and cytosolic free calcium concentration ([Ca2+]i) was determined. RT-PCR analysis revealed that the molecular identity of P2 purinoceptor localized to GMC was both P2U and P2Y. The rank order of potency in stimulating [Ca2+]i was ATP [symbol: see text] UTP > 2-methylthio-ATP > ADP [symbol: see text] adenosine [symbol: see text] AMP. In addition, cross-desensitization between ATP and UTP occurred. In conclusion, ATP induces increase in [Ca2+]i via P2U purinoceptor in GMC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate / pharmacology
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Adenosine Triphosphate / analogs & derivatives
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Adenosine Triphosphate / pharmacology*
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Animals
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Calcium / metabolism*
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Cells, Cultured
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DNA, Complementary / genetics
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Glomerular Mesangium / metabolism*
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Mice
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Polymerase Chain Reaction
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Receptors, Purinergic P2 / genetics
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Receptors, Purinergic P2 / metabolism*
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Receptors, Purinergic P2Y2
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Transcription, Genetic
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Uridine Triphosphate / pharmacology
Substances
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DNA, Complementary
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P2ry2 protein, mouse
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Receptors, Purinergic P2
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Receptors, Purinergic P2Y2
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Adenosine Diphosphate
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Adenosine Triphosphate
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Calcium
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Uridine Triphosphate