Abstract
The constitutive expression of interleukin-8 (IL-8) by human melanoma cells correlates with their metastatic potential. The exposure of human melanoma cells to the inflammatory cytokines IL-1 beta or tumor necrosis factor-alpha (TNF-alpha) upregulated IL-8 expression in a time-dependent and concentration-dependent manner. This enhanced expression of IL-8 was inhibited by cycloheximide or actinomycin-D. Treatment of melanoma cells with interferon (IFN) alpha, beta, or gamma did not affect the constitutive expression of IL-8, but IFN-alpha and IFN-beta blocked the upregulation of IL-8 expression in cells treated with IL-1 beta or TNF-alpha subsequent to or simultaneously with the IFN. These data suggest that the expression of IL-8 in human melanoma cells can be upregulated by inflammatory cytokines and that IFN-alpha and IFN-beta can counterregulate this stimulation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cycloheximide / pharmacology
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Dactinomycin / pharmacology
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Female
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Interferon alpha-2
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Interferon-alpha / pharmacology
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Interferon-beta / pharmacology*
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Interferon-gamma / pharmacology
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Interleukin-1 / pharmacology
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Interleukin-10 / pharmacology
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Interleukin-8 / biosynthesis*
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Interleukin-8 / genetics
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Lung Neoplasms / pathology
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Lung Neoplasms / secondary
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Lymphatic Metastasis / pathology
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Melanoma / genetics*
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Melanoma / pathology
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Melanoma / secondary
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Mice
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Mice, Nude
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Middle Aged
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Nucleic Acid Synthesis Inhibitors / pharmacology
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Protein Synthesis Inhibitors / pharmacology
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Recombinant Proteins / pharmacology
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Tumor Cells, Cultured
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Interferon alpha-2
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Interferon-alpha
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Interleukin-1
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Interleukin-8
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Neoplasm Proteins
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Nucleic Acid Synthesis Inhibitors
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Protein Synthesis Inhibitors
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Dactinomycin
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Interferon-beta
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Interferon-gamma
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Cycloheximide