Abstract
We expressed two regions of the serine repeat antigen (SERA) protein of Plasmodium falciparum in Escherichia coli by synthesizing the genes with a changed codon usage. One of the synthetic gene sequences encodes amino acid residues 17-382 (SE47') and the other encodes amino acid residues 586-802 (SE50A). The products produced by the synthetic gene sequences in E. coli accounted for 15-30% of the total bacterial protein. Antisera against both the purified gene products prepared in rats inhibited malaria parasite growth in vitro. The anti-SE47' serum was significantly more inhibitory than the anti-SE50A serum. The described methods provide a large scale preparation of recombinant antigens for improving and producing malaria vaccine.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Protozoan / immunology
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Antigens, Protozoan / biosynthesis
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Antigens, Protozoan / genetics*
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Base Sequence
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Escherichia coli / genetics*
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Escherichia coli / immunology
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Escherichia coli / metabolism
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Female
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Genes, Protozoan / immunology*
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Genes, Synthetic / immunology*
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Genetic Vectors / immunology
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Malaria, Falciparum / prevention & control
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Molecular Sequence Data
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Plasmodium falciparum / genetics
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Plasmodium falciparum / immunology
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Plasmodium falciparum / metabolism*
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Rats
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Rats, Sprague-Dawley
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Recombinant Proteins / biosynthesis*
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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Recombinant Proteins
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serine repeat antigen, Plasmodium