Abstract
We have cloned a cDNA that codes for a putative human UDP-galactose translocator (UGT) protein. The cDNA contained an open reading frame of 1,179 base pairs encoding a novel protein of 393 amino acids. Introduction of the open reading frame sequence into a UGT-deficient mouse cell line, Had-1, complemented the genetic defect of the mutant, namely the inability to transport UDP-galactose from the cytosol to the Golgi lumen, as judged from the lectin-sensitivity spectrum of the transformant. To our knowledge, this is the first mammalian nucleotide-sugar translocator whose cDNA sequence has been described.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Transport, Active
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Caenorhabditis elegans / genetics
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cell Line
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Cloning, Molecular
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DNA, Complementary / genetics*
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Genetic Complementation Test
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Golgi Apparatus / metabolism
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Humans
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Mice
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Molecular Sequence Data
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Monosaccharide Transport Proteins / chemistry
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Monosaccharide Transport Proteins / genetics*
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Monosaccharide Transport Proteins / metabolism
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Mutation
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Open Reading Frames
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Uridine Diphosphate Galactose / metabolism*
Substances
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Carrier Proteins
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DNA, Complementary
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Monosaccharide Transport Proteins
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Recombinant Proteins
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UDP-galactose translocator
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Uridine Diphosphate Galactose