Abstract
The purified recombinant V antigen from Yersinia pestis, expressed in Escherichia coli and adsorbed to aluminum hydroxide, an adjuvant approved for human use, was used to immunize outbred Hsd:ND4 mice subcutaneously. Immunization protected mice from lethal bubonic and pneumonic plague caused by CO92, a wild-type F1+ strain, or by the isogenic F1- strain C12. This work demonstrates that a subunit plague vaccine formulated for human use provides significant protection against bubonic plague caused by an F1- strain (C12) or against substantial aerosol challenges from either F1+ (CO92) or F1-(C12) Y. pestis.
MeSH terms
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Animals
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Antibodies, Bacterial / biosynthesis
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Antibodies, Bacterial / blood
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Antigens, Bacterial / immunology*
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Bacterial Capsules / analysis
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Enzyme-Linked Immunosorbent Assay
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Female
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Immunoglobulin G / biosynthesis
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Immunoglobulin G / blood
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Mice
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Plague / microbiology
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Plague / prevention & control*
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Plague Vaccine* / immunology
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Pore Forming Cytotoxic Proteins
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Vaccination
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Vaccines, Synthetic* / immunology
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Yersinia pestis / immunology*
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Immunoglobulin G
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LcrV protein, Yersinia
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Plague Vaccine
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Pore Forming Cytotoxic Proteins
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Vaccines, Synthetic