Abstract
Previous analysis of hybrid progeny derived from lipopolysaccharide (LPS) responder and nonresponder inbred mouse strains demonstrated that a single genetic locus controlled responsiveness to LPS. Using a differential functional screening approach, we report the isolation of a cDNA that has sequence homology to a GTP-binding protein. Expression of the cDNA in splenic B cells of C3H/HeJ nonresponder, endotoxin-resistant mice resulted in polyclonal B-cell activation in response to LPS stimulation. Thus a GTP-binding protein may be involved in LPS stimulation in B cells and perhaps other cell types.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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B-Lymphocytes / immunology*
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Base Sequence
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DNA, Complementary / genetics
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GTP-Binding Proteins / chemistry
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GTP-Binding Proteins / genetics*
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Gene Expression
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Lipopolysaccharides / immunology*
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Lymphocyte Activation*
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Mice
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Mice, Inbred C3H
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Molecular Sequence Data
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Molecular Weight
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Monomeric GTP-Binding Proteins*
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Sequence Homology, Nucleic Acid
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ran GTP-Binding Protein
Substances
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DNA, Complementary
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Lipopolysaccharides
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Ran protein, mouse
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GTP-Binding Proteins
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Monomeric GTP-Binding Proteins
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ran GTP-Binding Protein