Abstract
Wiskott-Aldrich syndrome is an X-linked combined immunodeficiency affecting cells of several different hemopoietic lineages. The Wiskott-Aldrich syndrome protein (WASP), which has no homology with any other known protein families, is rich in proline motifs known to contribute to Src homology 3 binding sites. However, its function has not been determined. The Tec family of cytoplasmic tyrosine kinases, which include Btk (the X-linked agammaglobulinemia gene), Itk, and Tec, is thought to be involved in lymphoid cell signaling pathways. In this work, we show binding of WASP to the Src homology 3 domains of Btk, Itk, Tec, Grb2, and phospholipase C-gamma, which suggests a function for WASP in lymphoid cell signaling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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B-Lymphocytes / metabolism*
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Binding Sites
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Burkitt Lymphoma / pathology
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Cell Differentiation
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Cell Line, Transformed
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Gene Expression
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Humans
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Molecular Sequence Data
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Peptide Fragments / metabolism
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Proline / chemistry
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Protein Binding
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Proteins / chemistry
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Proteins / physiology*
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Recombinant Fusion Proteins / metabolism
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Signal Transduction / physiology*
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Tumor Cells, Cultured
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Wiskott-Aldrich Syndrome / genetics*
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Wiskott-Aldrich Syndrome / immunology
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Wiskott-Aldrich Syndrome / pathology
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Wiskott-Aldrich Syndrome Protein
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src Homology Domains / physiology*
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src-Family Kinases / chemistry
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src-Family Kinases / metabolism*
Substances
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Peptide Fragments
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Proteins
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Recombinant Fusion Proteins
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WAS protein, human
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Wiskott-Aldrich Syndrome Protein
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Proline
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src-Family Kinases