Isolation of protein factors from opossum (Didelphis albiventris) serum which protect against Bothrops jararaca venom

Toxicon. 1996 Sep;34(9):1067-71. doi: 10.1016/0041-0101(96)00034-7.

Abstract

The fractionation of Didelphis albiventris serum by DEAE-Sephadex A50 yields a fraction (DA2) which protects the opossum against Bothrops venom. One polypeptide (DA2-II) responsible for this protection was isolated from fraction DA2 by ion exchange chromatography and biochemically characterized. DA2-II is a 43,000 mol. wt glycoprotein with the following N-terminal sequence: LKAMDTTPPLKIKKEPVK. Pairwise comparison of the amino acid sequence with four anti-hemorrhagic factors isolated from other opossum species indicated that DA2-II possesses high similarity (60-80%) with these proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antivenins / chemistry
  • Antivenins / isolation & purification
  • Antivenins / pharmacology*
  • Blood Proteins / chemistry
  • Blood Proteins / isolation & purification*
  • Blood Proteins / pharmacology
  • Bothrops
  • Chemical Fractionation
  • Chromatography, Ion Exchange
  • Crotalid Venoms / toxicity*
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Sequence Data
  • Molecular Weight
  • Opossums / blood*

Substances

  • Antivenins
  • Blood Proteins
  • Crotalid Venoms