Rapid detection and isolation of shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay

J Clin Microbiol. 1996 Nov;34(11):2812-4. doi: 10.1128/jcm.34.11.2812-2814.1996.

Abstract

By combining the enterohemolysin test and the VTEC-RPLA test (specific for the detection of Shiga-like toxin I [SLT-I], SLT-II, and SLT-IIc), single colonies of SLT-producing Escherichia coli were found to constitute between 0.03 and 68.1% of the coliform flora in human stool cultures and were isolated and characterized within 72 to 96 h.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agar
  • Animals
  • Bacterial Toxins / biosynthesis*
  • Bacteriological Techniques*
  • Blood
  • Colitis / diagnosis
  • Colitis / microbiology
  • Escherichia coli / isolation & purification*
  • Escherichia coli / metabolism*
  • Escherichia coli / pathogenicity
  • Escherichia coli Infections / diagnosis
  • Escherichia coli Infections / microbiology
  • Evaluation Studies as Topic
  • Feces / microbiology
  • Hemolysis
  • Hemolytic-Uremic Syndrome / diagnosis
  • Hemolytic-Uremic Syndrome / microbiology
  • Humans
  • Latex Fixation Tests / methods*
  • Sheep
  • Shiga Toxin 1

Substances

  • Bacterial Toxins
  • Shiga Toxin 1
  • Agar