Anti-glomerular basement membrane disease: role of enzyme-linked immunosorbent assays in diagnosis

Biochem Mol Med. 1996 Oct;59(1):52-6. doi: 10.1006/bmme.1996.0064.

Abstract

We compared two enzyme-linked immunosorbent assay (ELISA) tests with indirect immunofluorescence (IFA) for the measurement of anti-glomerular basement membrane (GBM) antibodies to study their sensitivity and specificity. The first ELISA test kit compared well to IFA (96.4% agreement, 93.3% sensitivity, 97.0% specificity). The second ELISA kit was much less sensitive (87.8% agreement, 63.3% sensitivity, 92.2% specificity). ELISA tests for the measurement of anti-GBM antibodies allow for quantitation and may have greater sensitivity than IFA. Sensitivity, however, may depend on the commercial test used.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Glomerular Basement Membrane Disease / diagnosis*
  • Antibodies / metabolism
  • Basement Membrane / immunology
  • Child
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Middle Aged

Substances

  • Antibodies