Disruption of light-induced c-Fos immunoreactivity in the suprachiasmatic nuclei of chronic epileptic rats

Neurosci Lett. 1996 Sep 27;216(2):105-8. doi: 10.1016/0304-3940(96)13020-2.

Abstract

Photic stimulation during specific day periods may induce Fos oncoprotein expression within the ventrolateral part of the suprachiasmatic nucleus (SCN) in the hypothalamus of rodents. This phenomenon appears to be a major molecular mechanism for environmental light/dark cycle entrainment of the mammalian circadian clock. Light-dependent synchronization of circadian rhythmicity may be disrupted in epilepsy, a chronic neurological disorder often associated with chronobiological features such as seizure periodicity and disruption of endogenous biological rhythms. The present work examined the light-induced Fos protein expression on the SCN in the pilocarpine model of chronic epilepsy. Fos-like immunoreactivity was significantly reduced in the SCN of chronic epileptic rats after photic stimulation during the subjective night. These results indicate an altered Fos protein expression in the SCN of chronic epileptic rats. The present findings reveal that pathological neural events underlying epileptogenesis may disturb circadian rhythm regulation. The experimental study of circadian clock activity in the SCN may clarify the molecular bases of chronobiological disturbances in epilepsy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Specificity
  • Benzoxazines
  • Chronic Disease
  • Circadian Rhythm / physiology
  • Coloring Agents
  • Epilepsy / metabolism*
  • Gene Expression Regulation / physiology
  • Genes, Immediate-Early / physiology
  • Immunohistochemistry
  • Light
  • Male
  • Muscarinic Antagonists / pharmacology
  • Oxazines
  • Pilocarpine / pharmacology
  • Proto-Oncogene Proteins c-fos / analysis*
  • Proto-Oncogene Proteins c-fos / genetics
  • Proto-Oncogene Proteins c-fos / immunology
  • Rats
  • Rats, Wistar
  • Suprachiasmatic Nucleus / chemistry*
  • Suprachiasmatic Nucleus / physiopathology

Substances

  • Benzoxazines
  • Coloring Agents
  • Muscarinic Antagonists
  • Oxazines
  • Proto-Oncogene Proteins c-fos
  • Pilocarpine
  • cresyl violet