Objective: To examine whether pigs with solid immunity against pseudorabies virus (PRV) could harbor latent infection with wild-type PRV without developing antibodies against glycoprotein E (gE), which is used as a marker protein to differentiate pigs that have been vaccinated from pigs infected with wild-type PRV.
Animals: Specific-pathogen-free pigs that were seronegative for antibodies to PRV.
Procedure: Oropharyngeal swab specimens were collected, and virus content was measured, Serum samples were obtained 1 week before and 2 and 4 weeks after challenge exposure. Four weeks after challenge exposure, pigs were slaughtered; various tissues were collected for virus isolation, and DNA was analyzed by polymerase chain reaction.
Results: Of the intranasally inoculated pigs, only 1 pig shed virus (for 1 day) but none developed antibodies directed against gE. Of the inoculated IM pigs, 3 pigs shed virus (for several days) and 5 developed antibodies directed against gE. One pig did not shed virus and did not develop detectable gE antibodies. However, variable amounts of wild-type virus DNA were detected in various tissues.
Conclusions: Immunized pigs can be infected with wild-type PRV without being detected by the gE-specific ELISA, which is routinely used to discriminate between infected and vaccinated pigs. The implication for eradication programs is that these pigs might be a source of new outbreaks.