N-(1,3-Dimethylbutyl)-N-phenylparaphenylenediamine (DMPPD) is a derivative of phenylenediamine (PPD) which is widely used in the rubber industry as an antioxidant. DMPPD which is a strong allergen, is least studied for its clastogenic potency. This study evaluated the genotoxic property of DMPPD in Swiss albino mice bone marrow cells by using chromosomal aberration and micronuclei assay. Three concentrations of DMPPD, i.e., 100, 150 and 200 mg/kg body wt. did not significantly increase the micronuclei in polychromatic erythrocytes or the ratio of poly to normochromatic erythrocytes. Chromosome aberration studies using 100 mg and 200 mg/kg body wt. showed that DMPPD is a non-inducer of chromosome aberrations. The results indicated non-clastogenicity of DMPPD in mice marrow cells up to a concentration of 200 mg/kg body wt. under our experimental conditions.