Abstract
Gellan lyase was purified from the culture fluid of soil samples incubated in a medium containing gellan as a sole carbon source. The enzyme was a monomer with a molecular mass of 140 kDa and was most active at pH 7.5 and 45 degrees C. The enzyme was highly specific to gellan and lowered the viscosity of the polymer.
MeSH terms
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Carbohydrate Sequence
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Hydrogen-Ion Concentration
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Molecular Sequence Data
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Molecular Weight
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Polysaccharide-Lyases / chemistry
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Polysaccharide-Lyases / isolation & purification*
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Polysaccharide-Lyases / metabolism
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Polysaccharides, Bacterial / chemistry
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Soil Microbiology
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Substrate Specificity
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Viscosity
Substances
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Polysaccharides, Bacterial
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gellan gum
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Polysaccharide-Lyases
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gellan lyase