Abstract
Monoclonal antibody BB1 was previously shown to recognize the CD80 protein as well as a putative B7.3 molecule, both ligands of the important T-cell receptors CD28 and CTLA-4. We report that BB1 coprecipitated from detergent lysates of human B- and pre-B-cell lines a protein kinase activity, as detected by solid phase immunoprecipitation followed by in vitro kinase assay. As other monoclonal antibodies to CD80 did not co-precipitate any kinase activity, it seems likely that this protein kinase is associated with the so far poorly characterized putative B7.3 molecule.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal
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Antigens, CD / analysis
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Antigens, CD19 / analysis
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Antigens, Surface / analysis*
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Antigens, Surface / isolation & purification
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Antigens, Surface / metabolism
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B-Lymphocytes
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B7-1 Antigen / analysis
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B7-1 Antigen / metabolism
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Cell Line
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Humans
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Phosphorylation
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Protein Kinases / analysis*
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Protein Kinases / isolation & purification
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Protein Kinases / metabolism
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, CD19
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Antigens, Surface
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B7-1 Antigen
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Protein Kinases