The turkey c-rap1A proto-oncogene is expressed via two distinct promoters

Gene. 1996 Oct 24;177(1-2):7-10. doi: 10.1016/0378-1119(96)00250-8.

Abstract

The turkey c-K-ras(B) transcript and two species of the turkey rap1A transcripts transcribed from two distinct promoters were isolated from a turkey spleen cDNA library. Turkey K-Ras and Rap1A proteins shared extensive amino acid (aa) sequence relatedness throughout their major functional domains: the four GTP-binding domains, the effector region and the C-terminal CAAX box. However, they diverged significantly in their intervening regions. In contrast, almost complete identity in the aa composition was exhibited between turkey K-Ras and Rap1A and their human homologues. The complete conservation that exists between turkey and human Rap1A, also along the polybasic C-terminal domain as opposed to Rap1B, suggests the functional relevance of these divergent residues in specifying the distinct biological functions of these two closely related proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • GTP-Binding Proteins / genetics*
  • Genes, ras*
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Proto-Oncogene Mas
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Turkeys
  • rap GTP-Binding Proteins

Substances

  • DNA, Complementary
  • MAS1 protein, human
  • Proto-Oncogene Mas
  • GTP-Binding Proteins
  • rap GTP-Binding Proteins

Associated data

  • GENBANK/X85754
  • GENBANK/X85755
  • GENBANK/X85756