[10 years' experiences with flow cytometric immunological phenotyping in malignant hematologic diseases]

Ugeskr Laeger. 1996 Oct 21;158(43):6098-102.
[Article in Danish]

Abstract

Immunological phenotyping of mononuclear cell suspensions from bone marrow and/or peripheral blood from 2341 patients (6140 examinations) suspected of malignant haematological diseases was performed during a 10-year period. The cells were labelled with a panel of monoclonal antibodies and subjected to flowcytometry. The results showed a clear distinction between acute lymphoblastic leukaemia and acute myeloid leukaemia, and a new group of acute hybrid leukaemia (3.3% of all acute leukaemia cases) was established. During the 10-year period immunological phenotyping has changed from being a research method to a widely employed method for cell characterisation early in the course of malignant haematological diseases.

Publication types

  • English Abstract

MeSH terms

  • Antibodies, Monoclonal
  • Diagnosis, Differential
  • Flow Cytometry*
  • Humans
  • Leukemia / diagnosis*
  • Leukemia / genetics
  • Leukemia / immunology
  • Lymphoma / diagnosis*
  • Lymphoma / genetics
  • Lymphoma / immunology
  • Monocytes / immunology
  • Myelodysplastic Syndromes / diagnosis*
  • Myelodysplastic Syndromes / genetics
  • Myelodysplastic Syndromes / immunology
  • Phenotype*
  • Prospective Studies
  • Retrospective Studies

Substances

  • Antibodies, Monoclonal