Abstract
The 3' ends of most eukaryotic messenger RNAs are generated by endonucleolytic cleavage and polyadenylation. In mammals, the cleavage and polyadenylation specificity factor (CPSF) plays a central role in both steps of the processing reaction. Here, the cloning of the 73-kilodalton subunit of CPSF is reported. Sequence analyses revealed that a yeast protein (Ysh1) was highly similar to the 73-kD polypeptide. Ysh1 constitutes a new subunit of polyadenylation factor I (PFI), which has a role in yeast pre-mRNA 3'-end formation. This finding was unexpected because in contrast to CPSF, PFI is only required for the polyadenylation reaction. These results contribute to the understanding of how 3'-end processing factors may have evolved.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Line
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Cloning, Molecular
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Evolution, Molecular
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Fungal Proteins / chemistry*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Humans
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Molecular Sequence Data
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Molecular Weight
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Poly A / metabolism
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Polynucleotide Adenylyltransferase / metabolism
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RNA Precursors / metabolism
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RNA Processing, Post-Transcriptional
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RNA, Fungal / metabolism
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RNA, Messenger / metabolism
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RNA-Binding Proteins / chemistry*
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism
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Sequence Homology, Amino Acid
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mRNA Cleavage and Polyadenylation Factors
Substances
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Fungal Proteins
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RNA Precursors
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RNA, Fungal
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RNA, Messenger
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RNA-Binding Proteins
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mRNA Cleavage and Polyadenylation Factors
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Poly A
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Polynucleotide Adenylyltransferase
Associated data
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GENBANK/U28374
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GENBANK/X95906
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GENBANK/Z73287