Pyrophosphate is a source of phosphoryl groups for Escherichia coli protein phosphorylation

B Duclos; E Vaganay; M Dadssi; A J Cozzone

doi:10.1111/j.1574-6968.1996.tb08555.x

Pyrophosphate is a source of phosphoryl groups for Escherichia coli protein phosphorylation

FEMS Microbiol Lett. 1996 Nov 15;145(1):49-54. doi: 10.1111/j.1574-6968.1996.tb08555.x.

Authors

B Duclos¹, E Vaganay, M Dadssi, A J Cozzone

Affiliation

¹ Institut de Biologie et Chimie des Protéines, Centre National de la Recherche Scientifique, Lyon, France.

PMID: 8931326
DOI: 10.1111/j.1574-6968.1996.tb08555.x

Abstract

Pyrophosphate can serve as a source of phosphoryl groups for the phosphorylation of E. coli proteins. The main target of such phosphorylation is a 49-kDa protein which is covalently modified at serine. The same phosphorylation pattern is obtained in the presence of ATP, which suggests that pyrophosphate can substitute for ATP for bacterial protein phosphorylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autoradiography
Bacterial Proteins / metabolism
Cell Extracts
Diphosphates / metabolism*
Escherichia coli / metabolism*
Molecular Weight
Phosphoproteins / metabolism*
Phosphorus Radioisotopes
Phosphorylation

Substances

Bacterial Proteins
Cell Extracts
Diphosphates
Phosphoproteins
Phosphorus Radioisotopes