Haematological and biochemical changes in horses competing in the Endurance Test (Phase T and D) of an advanced Horse Trial (HT, n = 22) and the Endurance Test (Phases A-D) of an advanced (CCI) 3-day-event (TD, n = 11) over a similar course on the same day were studied. Environmental conditions during the event were cool (5.5-11.1 degrees C). Blood samples were collected from the horses in each group the evening prior to the Endurance Test, within 60 s after, and 10 min after, completion of Phase D (cross-country jumping). The following were determined in the blood samples and compared between the 2 groups of horses: packed cell volume (PCV), serum total protein [TP], serum albumin [ALB], plasma lactate [lactate], serum total calcium [TCa], plasma ionised calcium [Ca+2], serum inorganic phosphate [PO4], plasma pH, plasma sodium [Na], plasma potassium [K], serum chloride [Cl], serum urea nitrogen [SUN], serum creatinine [Cr] and serum glucose concentrations and aspartate aminotransferase (AST) and creatine kinase (CK) activities. The PCV and [Cr] were higher in the TD group and approached significance (P = 0.063 and P = 0.057, respectively). The [TP], [ALB], [Na], glucose concentration and CK, and AST were significantly higher and [Cl] and [PO4] were significantly lower in the TD group after exercise when compared to the HT group. It was deduced from these data that the horses competing in the 3-day-event experienced greater fluid and electrolyte losses, reduced glomerular filtration, higher glycogenolysis and had greater leakage of enzymes from working muscles during competition than horses competing in the horse trial.