Effect of hypothalamic lesion or chemical axotomy on restitution of immunoreactivity in mice after cyclophosphamide administration

Int J Immunopharmacol. 1996 May;18(5):289-94. doi: 10.1016/0192-0561(96)00021-5.

Abstract

Splenocytes harvested from mice that underwent chemical axotomy (by 6-hydroxydopamine) or that had electric lesions in their anterior hypothalami demonstrated a significant decrease in their proliferative ability after concanavalin-A stimulation in vitro. In axotomized mice pre-treated with cyclophosphamide, faster restitution of the proliferative activity was observed on days 6-8 after the drug's administration. Splenocytes isolated from mice treated with 6-hydroxydopamine did not exhibit any suppressive activity, which is characteristic of the restitution period following administration of cyclophosphamide. These results indicate that the autonomic nervous system exert a direct effect on immunoreactivity and on processes which are responsible for restitution after cyclophosphamide-induced immunodisorders.

MeSH terms

  • Animals
  • Axons / physiology
  • Cyclophosphamide / pharmacology*
  • Denervation
  • Electric Stimulation
  • Hypothalamus / immunology*
  • Hypothalamus / physiology*
  • Lymphocyte Activation / drug effects
  • Male
  • Mice
  • Mice, Inbred CBA
  • Oxidopamine / pharmacology
  • Spleen / cytology

Substances

  • Oxidopamine
  • Cyclophosphamide