Immunogenic and protective response in mice immunized with a purified, inactivated, Dengue-2 virus vaccine prototype made in fetal rhesus lung cells

Am J Trop Med Hyg. 1996 Nov;55(5):504-10. doi: 10.4269/ajtmh.1996.55.504.

Abstract

The feasibility of a purified, inactivated vaccine (PIV) against dengue type 2 (DEN-2) virus was explored. Dengue-2 virus strain 16681 was used for producing a monotypic PIV. Virus adapted to fetal rhesus lung (FRhL-2) cells was harvested from roller bottle culture supernatant fluids, concentrated, and purified on sucrose gradients. Analysis of purified virus preparations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting showed primarily envelope (E) and premembrane (prM) antigens. These preparations had a purity, estimated from silver-stained gels, of approximately 70%, and a yield, based on recovery of virus and viral antigen, of 10-20%. The purified virus was inactivated with 0.05% formalin at 22 degrees C, or alternatively, with 7 mRads from a 60Co source. Vaccinated mice developed high titers of anti-DEN-2 virus neutralizing antibody and were partially protected from virus challenge. These results warrant further testing and development of PIVs for the other DEN virus serotypes.

MeSH terms

  • Animals
  • Antibodies, Viral / analysis
  • Antigens, Viral / immunology
  • Blotting, Western
  • Cells, Cultured / virology
  • Dengue / immunology*
  • Dengue / prevention & control*
  • Dengue Virus / immunology*
  • Dengue Virus / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Lung / cytology
  • Macaca mulatta
  • Mice
  • Mice, Inbred ICR
  • Neutralization Tests
  • Vaccines, Inactivated / immunology*
  • Viral Envelope Proteins / immunology
  • Viral Matrix Proteins / immunology

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Vaccines, Inactivated
  • Viral Envelope Proteins
  • Viral Matrix Proteins