Isolation and sequence determination of cDNA encoding mouse rab 4 and candidate approach for the beige mutation in mice

Biochem Mol Biol Int. 1996 Nov;40(4):647-51. doi: 10.1080/15216549600201243.

Abstract

The Chediak-Higashi syndrome is characterized by partial albinism and recurrent infections with giant granules in granulocytes. This syndrome has been proposed to have a defect in vesicular transport. Rab 4 is a member of a family of Ras-related small GTP-binding proteins, which has been mapped in the locus of the Chediak-Higashi syndrome. We isolated a full length cDNA of rab 4 from a cDNA library of mouse liver. The clone is 1428 base pairs (bp) in length and contains a 639 bp open reading frame encoding a polypeptide of 213 residues. The deduced amino acid sequence is highly homologous to rab 4 from rat and human. We analyzed rab 4 as a candidate gene of the beige mouse, but we could not find any change in the sequence of the coding region of rab 4 mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification*
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / genetics*
  • Genomic Library
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutagenesis*
  • Open Reading Frames
  • Rats
  • rab4 GTP-Binding Proteins

Substances

  • DNA, Complementary
  • GTP-Binding Proteins
  • rab4 GTP-Binding Proteins

Associated data

  • GENBANK/D86563