Selection and fine mapping of chromosome-specific cDNAs: application to human chromosome 1

Genomics. 1996 Dec 1;38(2):149-54. doi: 10.1006/geno.1996.0610.

Abstract

We have developed a methodology for identification and fine mapping of chromosome-specific transcripts. Combining digestion of DNA with different restriction enzymes, ligation to "bubble" linkers, and PCR amplification from Alu and "bubble" primers, we have synthesized human chromosome 1-specific sequences from DNA of a somatic cell hybrid, A9Neol. After hybridization to human fetal brain cDNA, we could efficiently capture chromosome 1-specific cDNAs. The cDNAs were sequenced and used as probes in hybridizations to high-density filters containing the arrayed CEPH Mega-YAC library and to the arrayed cDNA library from infant brain made by B. Soares, which has been extensively sequenced. By this approach we have been able to select chromosome 1-specific cDNAs, to map them to chromosome 1 YAC contigs, and to identify and map corresponding longer cDNAs and ESTs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • DNA, Complementary
  • Humans
  • Mice
  • Molecular Sequence Data
  • Sequence Analysis, DNA

Substances

  • DNA, Complementary

Associated data

  • GENBANK/Z78280
  • GENBANK/Z78281
  • GENBANK/Z78282
  • GENBANK/Z78283
  • GENBANK/Z78284
  • GENBANK/Z78285
  • GENBANK/Z78286
  • GENBANK/Z78287
  • GENBANK/Z78288
  • GENBANK/Z78289
  • GENBANK/Z78290
  • GENBANK/Z78291
  • GENBANK/Z78292
  • GENBANK/Z78293
  • GENBANK/Z78294
  • GENBANK/Z78295
  • GENBANK/Z78296
  • GENBANK/Z78297
  • GENBANK/Z78298
  • GENBANK/Z78299
  • GENBANK/Z78300

Grants and funding