pH-dependent actions of THIP and ZAPA on an ionotropic Drosophila melanogaster GABA receptor

Brain Res. 1996 Nov 11;739(1-2):335-8. doi: 10.1016/s0006-8993(96)00998-5.

Abstract

The actions of THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol) and ZAPA (Z-3-[(aminoiminomethyl)thio]prop-2-enoic acid) were tested on an ionotropic homo-oligomeric GABA receptor of Drosophila melanogaster. The amplitude of currents activated by THIP and ZAPA declined rapidly during agonist application and a rebound response was observed on washout. By correcting the pH shift induced by these acid salts, responses more typical of GABA agonists were seen. Less striking pH-dependence was observed in the case of GABA responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylates / pharmacology*
  • Animals
  • Drosophila melanogaster
  • Female
  • GABA Agonists / pharmacology*
  • Hydrogen-Ion Concentration
  • Isoxazoles / pharmacology*
  • Oocytes / metabolism
  • Receptors, GABA / biosynthesis
  • Receptors, GABA / genetics*
  • Recombinant Proteins / biosynthesis
  • Xenopus
  • gamma-Aminobutyric Acid / pharmacology

Substances

  • Acrylates
  • GABA Agonists
  • Isoxazoles
  • Receptors, GABA
  • Recombinant Proteins
  • gamma-Aminobutyric Acid
  • 3-((aminoiminomethyl)thio)-2-propenoic acid
  • gaboxadol